치주질환 예방 및 개선제로서 숲개밀 추출물의 효능
Efficacy of the extract of Brachypodium sylvaticum as a preventive and an improving agent of periodontal disease
문성희, 손주리, 신성진, 오승한, 김성환, 배지명,
소속 상세정보
문성희 ( Moon Seong-Hee ) - Wonkwang University College of Dentistry Department of Dental Biomaterials
손주리 ( Son Ju-Lee ) - Wonkwang Health Science University Department of Dental Hygiene
신성진 ( Shin Seong-JIn ) - Wonkwang University College of Dentistry Department of Dental Biomaterials
오승한 ( Oh Seung-Han ) - Wonkwang University College of Dentistry Department of Dental Biomaterials
김성환 ( Kim Seong-Hwan ) - Korea Research Institute of Chemical Technology
배지명 ( Bae Ji-Myung ) - Wonkwang University College of Dentistry Department of Dental Biomaterials
Abstract
본 논문에서는 치주질환 개선제로서 숲개밀(Brachypodium sylvaticum, BS) 추출물의 활용가능성을 확인하기 위해, 항균, 항염증 및 파골세포 분화 억제 활성에 관한 효능을 평가하였다. 숲개밀 추출물의 Porphyromonas gingivalis (P. gingivalis)의 성장 저해능과 항균의 지속성을 평가하였다. 숲개밀의 항염증 활성을 확인하기 위해 lipopolysaccharide (LPS) 처리에 의해 활성화된 RAW264.7 세포에서 전염증성 사이토카인의 분비량을 효소결합면역측정법(Enzyme-linked immunosorbent assay)으로 측정하였고, 산화질소의 생성능과 세포생존율을 측정하였다. 또한 Tartrate-resistant acid phosphatase (TRAP) 염색과 활성으로 파골세포 분화능을 평가하였다. 숲개밀 추출물은 P. gingivalis에 대해서 유의성있는항균활성 및 항균의 지속성을 나타내었다. 또한 숲개밀 추출물은 세포독성없이 전염증성 사이토카인인 tumor necrosis factor (TNF-α) 와 interleukin-6 (IL-6) 분비를 유의성있게 감소시켰고, 산화질소 생성능을 현저히 저해시키는 것을확인하였다. 또한, 숲개밀 추출물은 생쥐의 골수세포에서 얻어진 bone marrow macrophages (BMMs)에서의 파골세포로의분화를 세포독성 없이 억제하였다. 결론적으로 숲개밀 추출물은 항균, 항염 및 파골세포 분화 억제능을 보였고, 이는치주질환 치료 및 개선제의 유력한 후보 물질이 될 수 있다.
We evaluated the antibacterial, anti-inflammatory, and inhibitory effect of osteoclast differentiation of Brachypodium sylvaticum (BS) to find out the possibility of preventing periodontal disease. The inhibition of Porphyromonas gingivalis (P. gingivalis) growth by BS and the sustainability of the antibacterial activity was assessed. The production of pro-inflammatory cytokines from lipopolysaccharide (LPS)-induced RAW 264.7 cells were measured using enzyme-linked immunosorbent assays (ELISA), and the production of nitric oxide (NO) and cell viability were measured. Osteoclast differentiation was evaluated by Tartrate-resistant acid phosphatase (TRAP) staining, and TRAP activity. BS showed significant antibacterial activity and sustainable antibacterial activity in P. gingivalis. We also found out that the BS significantly decreased secretion of pro-inflammatory cytokines [tumor necrosis factor (TNF-α) and interleukin-6 (IL-6)] and NO production without cytotoxicity. Furthermore, BS inhibited the differentiation of bone marrow macrophages (BMMs) obtained from mouse bone marrow cells into osteoclasts without cytotoxicity. Taken together, BS can be a promising candidate for a preventive and improving agent of periodontal disease having antibacterial, anti-inflammatory, and inhibitory effects of osteoclast differentiation.
키워드
숲개밀; 치주질환; 항균; 항염; 파골세포분화
Brachypodium sylvaticum; Periodontal disease; Antibacterial activity; Anti-inflammation; Osteoclast differentiation
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